![]() Nucleic Acids Res.What advantage does the X10 have over other shafts? Are there any disadvantages? Mfold web server for nucleic acid folding and hybridization prediction. Fast insulin secretion reflects exocytosis of docked granules in mouse pancreatic B-cells. SUR1 regulates PKA-independent cAMP-induced granule priming in mouse pancreatic B-cells. Profound defects in pancreatic beta-cell function in mice with combined heterozygous mutations in Pdx-1, Hnf-1α, and Hnf-3β. Insulin secretion and differential gene expression in glucose-responsive and -unresponsive MIN6 sublines. Identification of tissue-specific microRNAs from mouse. Prediction of mammalian microRNA targets. V-1, a catecholamine biosynthesis regulatory protein, positively controls catecholamine secretion in PC12D cells. An amino-terminal domain of Mxi1 mediates anti-Myc oncogenic activity and interacts with a homolog of the yeast transcriptional repressor SIN3. Expression and distribution of lactate/monocarboxylate transporter isoforms in pancreatic islets and the exocrine pancreas. Interleukin-1β-induced rat pancreatic islet nitric oxide synthesis requires both the p38 and extracellular signal-regulated kinase 1/2 mitogen-activated protein kinases. ![]() V-1, a protein expressed transiently during murine cerebellar development, regulates actin polymerization via interaction with capping protein. The SNARE Vti1a-β is localized to small synaptic vesicles and participates in a novel SNARE complex. Computational identification of microRNA targets. 5′-AMP-activated protein kinase controls insulin-containing secretory vesicle dynamics. Tsuboi, T., da Silva Xavier, G., Leclerc, I. Patch-clamp characterisation of omatostatin-secreting cells in intact mouse pancreatic islets. Tight coupling between electrical activity and exocytosis in mouse glucagon-secreting alpha-cells. PLoS Biol 2, E98 (2004)īarg, S., Galvanovskis, J., Gopel, S. Sequence-specific inhibition of small RNA function. Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing. A uniform system for microRNA annotation. in Current Protocols in Molecular Biology (eds Ausubel, F. ![]() The Drosophila microRNA Mir-14 suppresses cell death and is required for normal fat metabolism. bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation. Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. Role of microRNAs in plant and animal development. An extensive class of small RNAs in Caenorhabditis elegans. An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans. ![]() Identification of novel genes coding for small expressed RNAs. Lagos-Quintana, M., Rauhut, R., Lendeckel, W. MicroRNAs: genomics, biogenesis, mechanism, and function. Thus, miR-375 is a regulator of insulin secretion and may thereby constitute a novel pharmacological target for the treatment of diabetes.īartel, D. Inhibition of Mtpn by small interfering (si)RNA mimicked the effects of miR-375 on glucose-stimulated insulin secretion and exocytosis. Myotrophin ( Mtpn) was predicted to be and validated as a target of miR-375. The mechanism by which secretion is modified by miR-375 is independent of changes in glucose metabolism or intracellular Ca 2+-signalling but correlated with a direct effect on insulin exocytosis. Here we show that overexpression of miR-375 suppressed glucose-induced insulin secretion, and conversely, inhibition of endogenous miR-375 function enhanced insulin secretion. To study the role of miRNAs in pancreatic endocrine cells we cloned and identified a novel, evolutionarily conserved and islet-specific miRNA ( miR-375). Several miRNAs in animals exhibit tissue-specific or developmental-stage-specific expression, indicating that they could play important roles in many biological processes 2, 3, 4. MicroRNAs (miRNAs) constitute a growing class of non-coding RNAs that are thought to regulate gene expression by translational repression 1. ![]()
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